Friday, January 22, 2016

Candy Electrophoresis Lab Analysis

Candy Electrophoresis Lab Analysis


1. When we analyzed the results of our gel, we found that none of our sample dyes contained different dyes than in the four reference dyes.  However, the dye band from our blue M &Ms was bigger than the Blue 1 reference dye. Also our green Mike & Ike band was much smaller than the yellow 6 reference dye. Our brown M&M had a multicolor band, with both red and blue. None of our dyes moved in the wrong direction. 
2. The Blue 1 reference dye would migrate similarly to the carminic acid, because they have similar structural characteristics.  The Red 40 reference dye would migrate similarly to the Betanin, because they both are bonded in similar patterns. The Blue 1 reference dye would migrate similarly to the Fast green FCF, because they are very very similar in their structure. The Red 40 reference dye would migrate similarly to the citrus red 2 because their structural characteristics are alike in many ways. 
 3. Dog food Manufacturers might put artificial colors in dog food to make the food look more appealing to the dog owners. 
5. The two factors which control the distance the colored dye solutions migrate are charge and size.
6. The electrical current helps move the dyes through the gel. 
7.  The small holes in the gel allow the smaller DNA strands to move quicker through tot he end with the positive charge. The bigger strands have a harder time going through. 
8. I expect the molecule which weighs 600 daltons to go the farthest, and then 1000, then 2000 (probably covering half the distance of the 1000) and then the 5000 very very far behind. 

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