Electrophoresis Virtual Lab
Link:Electrophoresis Virtual Lab
http://learn.genetics.utah.edu/content/labs/gel/
Make a prediction:
1. How do you think you could figure out the lengths of the strands in the tube of DNA?
By using Gel Electrophoresis, which we learned about previously in our vodcast.
Go through the simulation:
2. What is the process called in which we measure the DNA microscopically?
It is called Gel Electrophoresis.
3. What is the “gel”?
It like a Jello sponge with super small holes in it. It sorts the DNA strands.
4. Write down the step of gel electrophoresis
a. Make/set up gel.
b. Place DNA samples into the wells at the end of the gel.
c. Add an electrical current and run the gel.
d. Stain the DNA strands and analyze the results.
5. What does the current do the DNA samples?
The current makes the samples move towards the positive part of the gel.
6. What kinds of strand move quickly and further down the gel?
The smaller strands move quicker.
7. What kinds of strand move slower and lag behind?
It is called Gel Electrophoresis.
3. What is the “gel”?
It like a Jello sponge with super small holes in it. It sorts the DNA strands.
4. Write down the step of gel electrophoresis
a. Make/set up gel.
b. Place DNA samples into the wells at the end of the gel.
c. Add an electrical current and run the gel.
d. Stain the DNA strands and analyze the results.
5. What does the current do the DNA samples?
The current makes the samples move towards the positive part of the gel.
6. What kinds of strand move quickly and further down the gel?
The smaller strands move quicker.
7. What kinds of strand move slower and lag behind?
The bigger strands move slower.
8. What about the strand that are the same length?
Strands that are the same length move the same distance and speed.
9. What helps us see the DNA strand in the gel?
Staining the DNA strands helps us see them.
10. What are the ingredients to make a gel?
Make your Gel!
Powdered Agarose, buffer, flask, microwave, gel mold, and gel comb
11. Load the Gel with the DNA!
12. After you load the DNA sample into the tray,what is the next step?
The next step is to add an electrical current
13. How do you know current is running through the gel?
You know current is running through the gel because air bubbles will be coming up from the electrodes on either side of the gel.
14. After the gel is done,what must you do to it before you can analyze your results?
You must stain it before you can analyze it.
15. How long does this process take?
This process takes about 30 minutes.
16. What type of light do you use to view the gel? Is it safe and what precautions would you might need to use?
You use UV light. It can hurt your eyes, so it is necessary to wear goggles.
1. Take a screenshot of your gel and paste below.
8. What about the strand that are the same length?
Strands that are the same length move the same distance and speed.
9. What helps us see the DNA strand in the gel?
Staining the DNA strands helps us see them.
10. What are the ingredients to make a gel?
Make your Gel!
Powdered Agarose, buffer, flask, microwave, gel mold, and gel comb
11. Load the Gel with the DNA!
12. After you load the DNA sample into the tray,what is the next step?
The next step is to add an electrical current
13. How do you know current is running through the gel?
You know current is running through the gel because air bubbles will be coming up from the electrodes on either side of the gel.
14. After the gel is done,what must you do to it before you can analyze your results?
You must stain it before you can analyze it.
15. How long does this process take?
This process takes about 30 minutes.
16. What type of light do you use to view the gel? Is it safe and what precautions would you might need to use?
You use UV light. It can hurt your eyes, so it is necessary to wear goggles.
1. Take a screenshot of your gel and paste below.
18. Write your size estimates below:
a. Strand1_______6000_______
b. Strand2______3500_________
c. Strand3______1500__________
19. Could you list one reason why we would run a Gel electrophoresis on someone and explain your answer.
We could run Gel electrophoresis on someone to run a paternity test based on DNA fingerprinting. If the parents are the child’s biological parents, then the gel electrophoresis on his DNA will be a combination of theirs.
Relate and Review
Write at least 5 sentences summarizing the process of electrophoresis and relating to what you’ve learned before.
To figure out the lengths of pieces of DNA, we use gel electrophoresis. This process consists of four steps. First, we make the gel out of Powdered Agarose and a buffer, and set up the gel with the current. Second, we place our DNA samples inside the wells at one side of the gel (near the side with the negative current). Third, we add the electrical current to run the gel. Finally, we stain and analyze the strands. We can use this process for paternity testing, and much more.
a. Strand1_______6000_______
b. Strand2______3500_________
c. Strand3______1500__________
19. Could you list one reason why we would run a Gel electrophoresis on someone and explain your answer.
We could run Gel electrophoresis on someone to run a paternity test based on DNA fingerprinting. If the parents are the child’s biological parents, then the gel electrophoresis on his DNA will be a combination of theirs.
Relate and Review
Write at least 5 sentences summarizing the process of electrophoresis and relating to what you’ve learned before.
To figure out the lengths of pieces of DNA, we use gel electrophoresis. This process consists of four steps. First, we make the gel out of Powdered Agarose and a buffer, and set up the gel with the current. Second, we place our DNA samples inside the wells at one side of the gel (near the side with the negative current). Third, we add the electrical current to run the gel. Finally, we stain and analyze the strands. We can use this process for paternity testing, and much more.
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