pGLO Observations , Data Recording & Analysis

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB carpet gray gray - pGLO LB/amp 0 gray gray + pGLO LB/amp 104 gray gray + pGLO LB/amp/ara 1/2 carpet/ 64 gray green (glowing)

2.	What two new traits do your transformed bacteria have?
	My transformed bacteria glow in the dark and are resistant to ampicillin.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	I think about 90 bacteria, because E. coli are about 1-2 micro liters each, and there were 100 micro liters.
4.	What is the role of arabinose in the plates?
	The arabinose made the bacteria glow by inhibiting the promoter and allowing the DNA polymerase to read the GFP Gene.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Three current uses for GFP are to study cancer in mice, to remove malaria gene in mosquitoes, and to study the spread of HIV in cells.
6.	Give an example of another application of genetic engineering.

Crops that are resistant to herbicide.